Primer3: PCR primer design tool

Primer 3

Primer3 is a free online tool to design and analyze primers for PCR and real time PCR experiments. Primer3 can also select single primers for sequencing reactions and can design oligonucelotide hybridization probes. The online tool constitutes some important features like primer detection, cloning, sequencing and Primer listing.

Link: http://frodo.wi.mit.edu/